Neelam Perveen

Dedicated biochemist and molecular biologist with a profound understanding of cellular and molecular processes. Skilled in a variety of laboratory techniques and technologies, contributing to advancements in the understanding of biological systems. Adept at collaborative research and effective communication of complex scientific concepts. Seeking opportunities to apply my knowledge and skills in a dynamic research environment.Seeking a teaching position to apply my scientific expertise and passion for education in a school setting.

Expression Correlation of BER pathway genes (XRCC1 and PARP1) with Blood Cancer Patients (MS Thesis)

• Investigating the gene expression patterns of XRCC1, PARP1 and key genes in the Base Excision Repair (BER) pathway in 210 individuals diagnosed with various blood cancers.
• Employing advanced molecular biology techniques, including quantitative real-time PCR, comet assay and LORD Q-assay.
• Applied 2-delta delta Ct analysis method for accurate computation of mRNA levels.
• Contributes valuable insights into the molecular mechanisms underlying blood cancers, paving the way for improved diagnostic strategies and targeted therapeutic
  interventions.

Conclusion: Chemo therapeutics play a crucial role in disrupting the regular DNA repair cycle and decreasing the white blood cell count.

Bacterial Landscape of Everyday Eats: A Comprehensive Analysis of Microbial Contamination in Common Food Staples (BS Thesis)

• Antimicrobial resistance testing on Muller-Hinton agar.
• Identification and characterization based on biochemical tests.
• Employed antimicrobial discs (Benex Ltd) to assess drug sensitivity and resistance patterns of E. coli isolates.

Conclusion: Isolated E. coli strains exhibited resistance against commonly prescribed antibiotics.

Exploring homologous recombination repair and base excision repair pathway genes for possible diagnostic markers in hematologic malignancies.
Sumaira Fida Abbasi · Ishrat Mahjabeen · Neelam Perveen · Imama Qamar · Maria Fazal Ul Haq · Rabia Shafique · Nadia Saeed · Nida Sarosh
Ashraf · Mahmood Akhtar Kayani
doi.org/10.1007/s00438-023-02078-2

Abstract:
This study investigated the differential expression of homologous recombination repair (HRR) and base excision repair (BER) pathway genes in hematologic malignancies (HMs). Analyzing a cohort of 210 blood cancer patients, significant downregulation of RAD51, XRCC2, XRCC3, APEX1, FEN1, PARP1, and XRCC1 genes was observed. The study highlighted the diagnostic and prognostic potential of RAD51 and APEX1, with a significant association between downregulation of RAD51, XRCC3, and APEX1 and decreased patient survival. Additionally, DNA damage assessment revealed increased lesions in selected genes in blood cancer patients, emphasizing the role of these genes as diagnostic markers in HMs.

• Experienced in operating Mass Spectrometry and realtime PCR systems .
• Skilled in primer designing and its optimization using bioinformatics tools and the utilization of other databases like Ensemble, NCBI, PAST, IDT, Primer Quest, Uniprot, APE Program, OriginPro etc .
• Skilled in performing Centrifugation, Chromatography, Gel Electrophoresis, Flow Cytometry, ELISA, and Electron Microscope procedures.
• Adept in utilizing X-Ray Crystallography for structural analysis.
• Possess expertise in DNA and RNA extraction from blood and tissue samples using the Phenol-Chloroform method and TRIZOL extraction protocol. Proficient in performing PCR,encompassing gradient, conventional , and Real-time PCR , for primer optimization and product amplification.
• Skilled in cDNA synthesis and conventional PCR for gene expression analysis.
• Macromolecule blotting and probing techniques (Northern and Southern Blotting).
• Competent in statistical analysis, including One way ANOVA and χ2 test .
• Utilized standard microbial cultivation, fluorescence microscopy, and molecular biology techniques to study uncultured mixed communities of microorganisms.
• Experienced in culturing artificial medium for gram positive and gram negative bacterium.
• Proficient in performing biochemical tests.