Neelam Perveen

Dedicated biochemist and molecular biologist with a profound understanding of cellular and molecular processes.Skilled in a variety of laboratory techniques and technologies, contributing to advancements in the understanding of biological systems. Adept at collaborative research and effective communication of complex scientific concepts. Seeking opportunities to apply my knowledge and skills in a dynamic research environment.

Passionate about delving into cancer genetics, my research interests lie in exploring molecular mechanisms driving oncogenesis and identifying potential therapeutic targets. Leveraging my recent work, I aim to contribute to advancements in understanding the intricate genetic landscape of cancer for improved diagnostics and treatment strategies.

Expression Correlation of BER pathway genes (XRCC1 and PARP1) with Blood Cancer Patients (MS Thesis)

• Investigating the gene expression patterns of XRCC1, PARP1 and key genes in the Base Excision Repair (BER) pathway in 210 individuals diagnosed with various blood cancers.
• Employing advanced molecular biology techniques, including quantitative real-time PCR, comet assay and LORD Q-assay.
• Contributes valuable insights into the molecular mechanisms underlying blood cancers, paving the way for improved diagnostic strategies and targeted therapeutic interventions.

Conclusion: Chemo therapeutics play a crucial role in disrupting the regular DNA repair cycle and decreasing the white blood cell count.

Bacterial Landscape of Everyday Eats: A Comprehensive Analysis of Microbial Contamination in Common Food Staples (BS Thesis)

• Antimicrobial resistance testing on Muller-Hinton agar.
• Employed antimicrobial discs (Benex Ltd) to assess drug sensitivity and resistance patterns of E. coli isolates.
• Isolated E. coli strains exhibited resistance against commonly prescribed anti-biotic.

Exploring homologous recombination repair and base excision repair pathway genes for possible diagnostic markers in hematologic malignancies.

Sumaira Fida Abbasi · Ishrat Mahjabeen · Neelam Parveen · Imama Qamar · Maria Fazal Ul Haq · Rabia Shafique · Nadia Saeed · Nida Sarosh
Ashraf · Mahmood Akhtar Kayani

doi.org/10.1007/s00438-023-02078-2

Abstract:

This study investigated the differential expression of homologous recombination repair (HRR) and base excision repair (BER) pathway genes in hematologic malignancies (HMs). Analyzing a cohort of 210 blood cancer patients, significant downregulation of RAD51, XRCC2, XRCC3, APEX1, FEN1, PARP1, and XRCC1 genes was observed. The study highlighted the diagnostic and prognostic potential of RAD51 and APEX1, with a significant association between downregulation of RAD51, XRCC3, and APEX1 and decreased patient survival. Additionally, DNA damage assessment revealed increased lesions in selected genes in blood cancer patients, emphasizing the role of these genes as diagnostic markers in HMs.

• Proficient in manual RNA extraction using Trizol method.
• Skilled in cDNA synthesis and conventional PCR for gene expression analysis.
• Experienced in operating spectrophotometers and realtime PCR systems.
• Competent in statistical analysis, including One way ANOVA and χ2 test.
• Applied 2-delta delta Ct analysis method for accurate computation of mRNA levels.
• Experienced in culturing artificial medium for gram positive and gram negative bacterium.
• Proficient in performing biochemical tests including oxidase, catalase, simmon's citrate test and triple sugar iron test.

Dr. Ishrat Mahjabeen

Associate Professor of Bio Science

ishrat.mahjabeen@comsats.edu.pk

+923363763632

CUI Islamabad Campus

Prof. Dr. Mahmood A. Kayani

Professor of Bio Science

mkayani@comsats.edu.pk

+923215357981

CUI Islamabad Campus