· Biochemical testing.
· Staining techniques for bacterial identification.
· Phenotypic tests for quorum sensing detection.
· Antimicrobial susceptibility testing.
· Screening of Quorum Sensing genes in Pseudomonas aeruginosa through PCR technique.
Technical proficiency in Lab:
Molecular biology:
- DNA Extraction
- Alkaline lysis method
- Phenol-Chloroform method
· PCR
· Agarose Gel Electrophoresis
Microbiology:
· Media preparation for bacterial cultures
· Streaking
· Disc Diffusion
· Well Diffusion
· MIC , Sub-MIC
· Biofilm Formation and biofilm assays
· Serial dilution for bacterial cultures
· Pouring Plugging slant preparation and streaking on plates
· Isolation and culturing of microbes from water sample
· Biochemical test for screening of microbes
· Thin layer and Paper chromatography
Equipment handled:
· Autoclave
· PCR
· Centrifuge
· Water Bath
· Biosafety Cabinet and Laminar Flow hood
· Spectrophotometer
· Incubator